P-030 Clinical outcomes of microdissection testicular sperm extraction (micro TESE) and intracytoplasmic sperm injection (ICSI) in non-obstructive azoospermia (NOA) with the history of cryptorchidism

Abstract Study question What is sperm retrieval rate (SRR) by micro TESE and the clinical outcomes using testicular in couples with history of cryptorchidism? Summary answer NOA cryptorchidism had a higher SRR but lower ICSI compared unexplained NOA. known already Undescended testis (UT) which exposed to temperature scrotal associated impairment germ cell maturation, progressive Leydig Sertoli atrophy, subsequent infertility adulthood. There have been very few studies focus on, or large enough numbers examine, specific male factor infertility, however improvement techniques including micromanipulation techniques, such as ICSI, has led excellent fertilization pregnancy treatment cycles. design, size, duration We performed retrospective study based on two reproduction centers Japan evaluated 1521 azoospermic patients our clinics between September 2013 December 2022. investigated these therefore aimed evaluate prevalence significance embryonic development cryptorchidism. Participants/materials, setting, methods TESE, pronuclei (2PN) oocyte rates, blastocyst development, good-quality (Grade 3BB above day 5 Gardner scoring), rates per embryo transfer (ET) 72 cases UT, 953 NOA, not after orchidopexy, Klinefelter syndrome, cryptozoospermia, mumps orchitis, 284 obstructive azoospermia (OA). Statistical analysis was unpaired t-tests chi-squared tests. Main results role chance first attempt UT (44/56=78.6%) than (160/748=21.4%) (p < 0.001). Spermatozoa were successfully retrieved 13 22 (59.1%) group 36 226 (15.9%) who previously undergone no found. No correlation found serum FSH, LH, T level success retrieval. Testicular volume patient age at orchidopexy also did affect for TESE. 2PN oocytes, blastocysts 45.5%, 47.4%, 18.9% 51.2%, 45.0%, 20.9% 62.6%, 52.1%, 23.7% OA, respectively. Clinical ET 29.3% 32.3% 41.6% OA. Significant differences only observed oocytes OA 0.05) 0.05), 0.01). Several showed small number spermatozoa even immotile Limitations, reasons caution included surgery cryptorchidism, delayed descent without surgery. The cohort size this small, however, screened population may be biased. Wider implications findings This shows high impact Our emphasizes that provides clinically valuable prognostic information considering surgical Trial registration applicable